Pascal Touzet

Pascal Touzet

Professeur des Universités 1ère classe

Directeur-adjoint de l’UMR CNRS 8198





Unité Evo-Eco-Paléo (EEP) – UMR 8198
CNRS / Université de Lille

Cité scientifique
Batiment SN2, bureau 102
59655 Villeneuve d’Ascq – FRANCE
Tel : 33 (0)3-20-43-40-32


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As an evolutionary geneticist, I have been studying the evolutionary and genetic factors that are involved in the maintenance or transition of plant mating systems and their impact on genome diversity. I focused mainly on systems with sex phenotype polymorphisms: gynodioecy in Beta vulgaris, gynodioecy and dioecy in Silene.

The study of S. nutans in collaboration with Fabienne Van Rossum (Botanic Garden, Meise Belgium) led us to the discovery of several differentiated lineages that exhibit very strong post-zygotic barriers to reproduction. We are currently using population genomics approaches to disentangle the evo-demographic scenarii of this speciation events as well as the cytonuclear genetic incompatibilities that are most likely involved.



Statistics, Evolutionary biology in Polytech’lille


Short  CV

01/2020 Deputy director of Unit. Evo-Eco-Paleo, UMR CNRS 8198

09/2016 Professor 1rst class, Univ. Lille, Polytech’Lille

2015-2019 Head of the research team Evolution Ecologie of UMR 8198

09/2010 Professor 2nd class, Univ. Lille, Polytech’Lille

11/2005 Habilitation à Diriger des Recherches en Sciences Naturelles, Univ. Lille 1.

02-07/2005 Visiting Professor in Prof. Lynda Delph’s lab, Indiana University, Bloomington, Etats-Unis (Bourse Fulbright/Région Nord-Pas de Calais)

1997-2010 Assistant Professor, Univ. Lille 1

1996/1997 Temporary lecturer, univ. Lille 1

1995/1996 Post-doc at Station de Génétique Végétale, INRA-UPS-INAP-G, Ferme du Moulon, Gif-sur-Yvette (Grant from  la Société de Secours des Amis des Sciences)

1995 PhD in Genetics of INAP-G, Station de Génétique Végétale (Gif-sur-Yvette) supervised by Prof. Dominique de Vienne

1993-1994 Visitor scientist in Prof. Tim Helentjaris’ lab, University of Arizona, Tucson, Etats-Unis

1990 Engineer Diploma in Agronomy of  INAP-G, Master in Plant Breeding


List of publications:

Submitted papers

– Muyle A*, Martin H*, Zemp N, Mollion M, Gallina S, TavaresR , Silva A, Bataillon T, Widmer A,  Glémin S, Touzet P#, Marais GAB#. Dioecy is associated with high genetic diversity and adaptation rates in the plant genus Silene.  * co-first authors; # co-senior authors (doi:

– Hartmann FE, Alodie Snirc A,  Cornille A, Godé C, Touzet P, Van Rossum F, Fournier E,  Le Prieur S, Shykoff J, Giraud T.  Congruent population genetic structures and demographic histories in anther-smut fungi and their host plants Silene nutans and S. italica.

– Vanderplanck M, Touzet P, Van Rossum F, Lahiani E, De Cauwer I , Dufaÿ M. Does pollination syndrome reflect pollinator efficiency in Silene nutans?


Published papers

* Master or PhD Students I supervised

  1. Varré J-S#, D’Agostino N#, Touzet P, Gallina S, Tamburino R, Cantarella C, Ubrig U,  Cardi T, Drouard L, Gualberto JM, Scotti N (2019) Complete sequence, multi-chromosomal architecture and transcriptome analysis of the Solanum tuberosum mitochondrial genome. Int. J. Mol. Sci. 20, 4788; doi:10.3390/ijms20194788 # co-premiers auteurs
  2. Martin H*, Carpentier F*, Gallina S, Godé C, Schmitt E, Muyle A, Marais GAB, Touzet P (2019) Evolution of young sex chromosomes in two dioecious sister plant species with distinct sex determination systems. Genome Biology and Evolution 11(2), 350–361. doi:10.1093/gbe/evz001
  3. Van Rossum F, Martin H*, Le Cadre S, Brachi B*, Christenhusz M, Touzet P (2018) Phylogeography of a widely distributed species reveals a cryptic assemblage of distinct genetic lineages needing separate conservation strategies. Perspectives in Plant Ecology, Evolution and Systematics 35, 44–51
  4. Meyer EH, Lehmann C, Boivin S*, Brings L, De Cauwer I , Bock R, Kühn K, Touzet P (2018) CMS-G from Beta vulgaris ssp. maritima is maintained in natural populations despite containing an atypical cytochrome c oxidase. Biochemical Journal 475, 759–773
  5. Touzet P, Villain S*, Buret L*, Martin H*, Holl A-C, Poux C, Cuguen J (2018) Chloroplastic and nuclear diversity of wild beets at a large geographical scale: toward an evolutionary history of the Beta section. Ecology and Evolution 8:2890–2900,
  6. Martin H*, Touzet P, Dufay M, Godé C, Schmitt E, Lahiani E*, Delph LF, Van Rossum F (2017) Lineages of Silene nutans developed rapid, strong, asymmetric post-zygotic reproductive isolation in allopatry. Evolution 71, 1519-153
  7. Martin H*, Touzet P, Van Rossum F, Delalande D*, Arnaud J-F (2016) Phylogeographic pattern of range expansion provides evidence for cryptic species lineages in Silene nutans in Western Europe. Heredity 116, 286-294 (3,804)
  8. Van Rossum F, Weidema IR, Martin H*, Le Cadre S, Touzet P, Prentice HC, Philipp M (2016) The structure of allozyme variation in Silene nutans (Caryophyllaceae) in Denmark and in north-western Europe. Plant Syst. Evol. 302, 23–40 (1,422)
  9. Lahiani E*, Touzet P, Billard E, Dufay M (2015) When is it worth to be a self-compatible hermaphrodite? Context-dependent effects of self-pollination on female advantage in gynodioecious Silene nutans. Ecology & Evolution 5, 1854–1862.
  10. Touzet P, Meyer E (2014) Cytoplasmic Male Sterility and mitochondrial metabolism in plants. Mitochondrion 19, 166-171
  11. Godé C, Touzet P, Martin H*, Lahiani E*, Delph L, Arnaud J-F (2014) Characterization of 24 polymorphic microsatellite markers for Silene nutans, a gynodioecious-gynomonoeicous species, and cross-species amplification in Silene species. Conservation Genetics Resources 6, 915–918
  12. Lahiani E*, Dufaÿ M, Castric V, Le Cadre S, Charlesworth D, Van Rossum F, Touzet P (2013) Disentangling the effects of mating systems and mutation rates on cytoplasmic diversity in gynodioecious Silene nutans and dioecious Silene otites. Heredity 111, 157–164
  13. Touzet P (2012) Mitochondrial genome evolution and gynodioecy. Advances in Botanical Research 63, 71–98
  14. Darracq A*, Varré JS, Maréchal-Drouard L, Courseaux A, Castric V, Saumitou-Laprade P, Oztas S, Lenoble P, Vacherie B,  Barbe V, Touzet P (2011) Structural and content diversity of mitochondrial genome in beet: a comparative genomic analysis. Genome Biology and Evolution 3, 723–736
  15. Garraud C, Brachi B*, Dufaÿ, Touzet P, Shykoff J (2011) Genetic determination of male sterility in gynodioecious Silene nutans. Heredity 106, 757–764
  16. Darracq A*, Varré J-S, Touzet P (2010) A scenario of mitochondrial genome evolution in maize based on rearrangement events. BMC Genomics 11, 233
  17. Arnaud J-F, Fénart S, Godé C, Deledicque S, Touzet P, Cuguen J (2009) Fine-scale geographical structure of genetic diversity in inland wild beet populations. Mol. Ecol. 18, 3201-3215
  18. Dufaÿ M, Cuguen J, Arnaud J-F, Touzet P (2009) Sex ratio variation among gynodioecious populations of wild beet: can it be explained by negative frequency-dependent selection? Evolution 63, 1483-1497
  19. Touzet P, Delph LF (2009) The effect of breeding system on polymorphism in mitochondrial genes of Silene. Genetics 181, 631-644
  20. Dufaÿ M, Vaudey V, de Cauwer I, Touzet P, Cuguen J, Arnaud J-F (2008) Variation in pollen production and pollen viability in natural populations of gynodioecious Beta vulgaris ssp. maritima: evidence for a cost of restoration? Journal of Evolutionary Biology 21, 202-212
  21. Laurent V, Devaux P, Thiel T, Viard F, Touzet P, Quillet MC (2007) Comparative effectiveness of sugar beet microsatellite markers isolated from genomic libraries and GenBank ESTs to map the sugar beet genome. Theoretical and Applied Genetics. 115, 793-805
  22. Mower JP, Touzet P, Gummow JS, Delph LF, Palmer JD (2007) Extensive variation in synonymous substitution rates in mitochondrial genes of seed plants. BMC Evolutionary Biology 7, 135 (highly accessed article, evalué par la Faculty of 1000 Biology)
  23. Dufaÿ M, Touzet P, Maurice S, Cuguen J (2007) Modelling the maintenance of a male fertile cytoplasm in a gynodioecious population. Heredity 99, 349-356
  24. Fievet V*, Touzet P, Arnaud J-F, Cuguen J (2007) Spatial analysis of nuclear and cytoplasmic DNA diversity in wild sea beet (Beta vulgaris ssp. maritima) populations: do marine currents shape the genetic structure? Molecular Ecology 16, 1847-1864
  25. Delph LF, Touzet P, Bailey MF (2007) Merging theory and mechanism in studies of gynodioecy. Trends in Ecology and Evolution 22, 17-24
  26. Roux F, Touzet P, Cuguen J, Le Corre V (2006) How to be early-flowering: An evolutionary perspective. Trends in Plant Science. 11, 375-381
  27. Fénart S, Touzet P, Arnaud J-F, Cuguen J (2006) Emergence of gynodioecy in wild beet (Beta vulgaris ssp. maritima L.): a genealogical approach using chloroplastic nucleotide sequences. Proceedings of the Royal Society of London, Series B 273, 1391-1398
  28. Touzet P, Budar F (2004) Unveiling the molecular arms race between two conflicting genomes in Cytoplasmic Male Sterility? Trends in Plant Science 9, 568-570
  29. Touzet P , Hueber N*, Burkholz A, Barnes S, Cuguen J (2004) Genetic analysis of male fertility restoration in wild cytoplasmic male sterility G of beet. Theoretical and Applied Genetics 109, 240-247
  30. Budar F, Touzet P, De Paepe R (2003) The nucleo-mitochondrial conflict in cytoplasmic male sterilities revisited. Genetica 117, 3-16
  31. Touzet P. (2002) Is rf2 a restorer gene of CMS-T in maize? Trends in Plant Science 7, 434
  32. Ducos E*, Touzet P, Boutry M (2001) The male sterile G cytoplasm of wild beet displays modified mitochondrial respiratory complexes. The Plant Journal. 26, 171-180
  33. Ducos E*, Touzet P, Saumitou-Laprade P,  Vernet P, Cuguen J (2001) Nuclear effect on mitochondrial protein expression of the CMS Owen cytoplasm in sugar beet. Theoretical and Applied Genetics, 102, 1299-1304
  34. Touzet P, Riccardi F, Morin C, Damerval C, Huet J-C, Pernollet J-C, Zivy M, de Vienne D. (1996) The maize two-dimensional gel protein database: towards an integrated genome analysis program. Theoretical and Applied Genetics 93, 997-1005
  35. Touzet P, de Vienne D, Huet J-C, Ouali C, Bouet F, Zivy M (1996) Amino acid analysis of proteins separated by two-dimensional electrophoresis in maize: isoform detection and function identification. Electrophoresis 17, 1393-1401
  36. Touzet P, Morin C, Damerval C, Le Guilloux M, Zivy M, de Vienne D (1995) Characterizing Allelic Proteins for Genome Mapping in Maize. Electrophoresis 16, 1289-1294
  37. Touzet P, Winkler RG, Helentjaris T (1995) Combined Genetic and Physiological Analysis of a Locus Contributing to Quantitative Variation. Theoretical and Applied Genetics 91, 200-205. (3,507)
  38. de Vienne D, Josse J-M, Maurice A, Causse M, Leonardi A, Touzet P, Krejci E, Gouesnard B, Sanou J, Panouille A, Boyat A, Dubreuil P, Dufour P, Gallais A, Lefort M, Charcosset A, Damerval C. (1994) Marquage et expression du génome chez le maïs. Genetics, Selection and Evolution 26, Suppl1, 21s-34s. Article original